An Immunoelectron Microscopic Comparison of Desmosomal Constituents and Hemidesmosomal Ones Originating from the Same Tissue of the Same Animal

Cell Structure and Function

Hisato Shida²  Malcolm S. Steinberg¹ 

[1] Department of Biology, Princeton University;Department of Biology, University of Yamanashi Medical School

关键词: desmosome;    hemidesmosome;    immunoelectron microscopy;    protein A-gold;    epithelial cells;   

DOI  :  10.1247/csf.16.175

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(24)Cited-By(2)The molecular constituents of desmosomes and hemidesmosomes were compared by examining bovine muzzle epidermis under immunoelectron microscopy using a postembedding method, first with antibodies prepared to four desmosomal antigens (DP1/2, DP3, DG1, DG2/3), followed by protein A-gold (PAG) complexes. The four antibodies showed almost negative labeling at hemidesmosomes as compared with the labeling observed at the desmosomes in the same tissue. By counting the number of PAG particles/200 mμ at hemidesmosomes and desmosomes, the above qualitative observation was confirmed quantitatively. These results support a new concept which has recently been proposed by several researchers that hemidesmosomes and desmosomes are immunochemically distinct.

【 授权许可】

Unknown