Cloning and Sequencing of Viral Integration Site in Human Fibroblasts Immortalized by Simian Virus 40

Cell Structure and Function

Osamu Yano²  Yuji Karasaki²  Hideyasu Hirano²  Ken Higashi²  Shinobu Akiya¹  Hayato Nakamura²  Sadao Gotoh² 

[1] Department of Ophthalmology, University of Occupational and Environmental Health;Department of Biochemistry, University of Occupational and Environmental Health

关键词: SV40;    human fibroblast;    integration;    immortalization;   

DOI  :  10.1247/csf.16.63

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(50)Cited-By(2)We have analyzed cellular DNA sequences at the viral genome integration site in a human fibroblast cell line VA13 immortalized by simian virus 40 (SV40). The computer analysis of the junctional cellular DNA sequences did not show any homology to the DNA sequences previously reported. This suggests that immortalization by SV40 was not induced by the destruction of any known oncogene or antioncogene at the integration site. We did not find the precise substantial sequence homology at the junctional site between the cellular DNA and SV40 DNA, indicating that the recombination mechanism involved does not require precise sequence homology and therefore, SV40 genome was probably not integrated by homologous recombination. Short direct and inverted repeats of 5 to 29 nucleotides were found in the junctional cellular and SV40 DNA. Cellular DNA abutting SV40 DNA was found by the Northern blot analysis to be expressed in diploid human fibroblasts and SV40-transformed cells. The nature of this RNA is now under study.

【 授权许可】

Unknown