Purification of DNA Ligases from Mouse Testis and their Behavior during Meiosis.

Cell Structure and Function

Atsushi Higashitani¹  Yasuo Hotta¹  Hideki Endo¹  Satoshi Tabata¹ 

[1] Biological Institute, School of Science, Nagoya University

关键词: DNA ligase;    meiosis;    mouse spermatocytes;   

DOI  :  10.1247/csf.15.67

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(22)Cited-By(2)Two types of DNA ligase, I and II, have been purified approximately 4, 000-fold from mouse testes and 500-fold from nuclei of mouse spermatocytes. DNA ligase I and II consisted of single polypeptides with molecular weights of 95, 000 and 65, 000, respectively, according to the estimation by SDS-polyacrylamide gel electrophoresis and the AMP-binding assay. Ligase activities were higher in premeiotic spermatogonia and spermatocytes than those in liver and bone marrow cells. Moreover, DNA ligase II showed rapid increase during meiotic prophase and a decrease in round spermatids. Since this behavior of DNA ligase II is consistent with that of m-rec and DNA polymerase β, both of which have been shown to be involved in DNA recombination in meiotic cells, DNA ligase II might be an enzyme which works at the final step of meiotic recombination reaction.

【 授权许可】

Unknown