A Simple Instrument for Luminescence/Growth Measurement for Bioluminescent Bacteria

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Hiroshi Kato¹  Satoshi Sasaki¹ 

[1] Graduate School of Biotechnology and Biosciences, Tokyo University of Technology

关键词: Oxygen;    Photobacterium kishitanii;    Bioluminescence;    Oscillation;    Growth;   

DOI  :  10.1252/jcej.16we088

来源: Maruzen Company Ltd

【 摘 要 】

References(21)The present study examines the relationship between the cell growth rate and luminescence intensity by fabricating a system that enables the simultaneous measurement of the luminescence intensity and optical density of the liquid culture of Photobacterium kishitani, in order to investigate a possible mechanism for the luminescence oscillation. An instrument is fabricated using a microcomputer-controlled LED and a photo IC diode for the measurements. Two signals, cell density and bacterial bioluminescence were distinguished from one data set. A possible hypothesis for the oscillation in bacterial bioluminescence from oxygen level viewpoint has been created.

【 授权许可】

Unknown