Protein-Binding Characteristics of Anion-Exchange Particles Prepared by Radiation-Induced Graft Polymerization at Low Temperatures

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Daisuke Umeno¹  Goro Furumoto²  Naohiro Shinohara²  Noboru Kubota²  Yuichi Shimoda¹  Hironobu Shirataki²  Yuta Sekiya¹  Kyoichi Saito¹ 

[1] Department of Applied Chemistry and Biotechnology, Chiba University;Microza & Water Processing Division, Asahi Kasei Chemicals Corp.

关键词: Protein Adsorption;    Anion Exchange;    Particles;    Radiation-Induced Graft Polymerization;    Glycidyl Methacrylate;   

DOI  :  10.1252/jcej.13we056

来源: Maruzen Company Ltd

【 摘 要 】

References(15)An epoxy-group-containing vinyl monomer, i.e., glycidyl methacrylate, was graft-polymerized onto polyethylene particles with an average diameter of 35 µm at various reaction temperatures within 278–333 K. The produced epoxy group was converted into a diethylamino group as an anion-exchange group. From the equilibrium binding capacity of the resultant particle-packed bed for bovine serum albumin (BSA) and the pressure loss required for a protein solution to flow through the bed, the formation of graft chains that were sufficiently long to hold BSA in multilayers and allow convective flow among them was predicted. The achieved ideal adsorption characteristics enabled a higher flow rate of the protein solution, which leads to a higher overall adsorption rate of the protein because of convective flow among the graft chains.

【 授权许可】

Unknown