Reactivity and Stability of Catalase-Bound Liposomes Containing Glucose Oxidase in an Airlift Bubble Column

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Makoto Yoshimoto¹  Masahiro Inoue¹  Noriyuki Takaki¹ 

[1] Department of Applied Molecular Bioscience, Yamaguchi University

关键词: External Loop Airlift;    Bubble Column;    Liposomes;    Glucose Oxidase;    Catalase;   

DOI  :  10.1252/jcej.12we051

来源: Maruzen Company Ltd

【 摘 要 】

References(13)Cited-By(1)Catalase-bound liposome containing glucose oxidase (CLG) has been used to catalyze the oxidation of glucose in an external loop airlift bubble column. In a static liquid system suspending CLG, glucose was steadily oxidized at 40°C at the initial pH of 7.4 for 73 h, although the oxidation rate is significantly small because of strong permeation resistance of the CLG membrane to glucose. In the airlift, the rate of oxidation catalyzed by the CLG with initial mean diameter DP of 324.9 nm significantly increases at superficial gas velocity UG of 0.5–3.0 cm/s compared to that in the static liquid. The gas–liquid flow has thus been found to induce permeabilization of the CLG. The reactivity of CLG in the airlift is influenced by the interaction between catalase (CA) and the membrane. Furthermore, the structural stability of CLG in the airlift is dependent on its initial DP. The CLG with DP of 168.8 nm remains practically unchanged in its size in the airlift at UG of 1.0 cm/s. On the other hand, the CLG with DP of 269.5 nm shows to be partly disrupted with deactivation of CA under the above condition. The reactivity and stability of CLG have also been elucidated in catalyzing the fed-batch oxidation of glucose for 34 h in the airlift.

【 授权许可】

Unknown