Enhancement of the Activity of a Lactobacilli-Aggregating Peptide by Freezing Treatment

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Mina Okochi²  Ryuji Kato²  Kyuya Nakagawa¹  Yuji Asai²  Hiroyuki Honda²  Tomoya Sugita² 

[1] Department of Mechanical and System Engineering, University of Hyogo;Department of Biotechnology, Graduate School of Engineering, Nagoya University

关键词: Lactobacillus rhamnosus;    Peptides;    Cell Aggregation;    Freezing;   

DOI  :  10.1252/jcej.12we086

来源: Maruzen Company Ltd

【 摘 要 】

References(20)The effects of freezing treatments on the activity of the lactobacilli-aggregating peptide QRCVNLQA were studied. The aggregation activity of the peptide solution was enhanced by rapid deep freezing in liquid nitrogen followed by storage for at least 1 d below －10°C. The treated peptide strongly induced the aggregation of suspended cells of Lactobacillus rhamnosus GG to provide a clear supernatant after 1.5 h. The peptide was activated by oxidation of thiol groups, probably through dimerization of the peptide molecules to form disulfide bonds. Addition of the freeze-oxidized peptide to a suspension of lactobacilli promoted the adhesion of the lactobacilli to a monolayer of Caco-2 intestinal epithelial cells.

【 授权许可】

Unknown