JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | |
Hollow Fiber Bioreactor Perfusion Culture System for Magnetic Force-Based Skeletal Muscle Tissue Engineering | |
Hideaki Jitsunobu1  Katsuya Yamaguchi2  Masamichi Kamihira2  Yoshinori Kawabe1  Akira Ito1  Hiroshi Mizumoto2  Yasunori Yamamoto2  | |
[1] Department of Chemical Engineering, Faculty of Engineering, Kyushu University;Graduate School of Systems Life Sciences, Kyushu University | |
关键词: Skeletal Muscle; Hollow-Fiber Bioreactor; Perfusion Culture; Magnetite Cationic Liposome; C2C12 Cell; | |
DOI : 10.1252/jcej.11we237 | |
来源: Maruzen Company Ltd | |
【 摘 要 】
References(21)Cited-By(2)Large-scale skeletal muscle tissue cultures are often limited by nutrient supplementation and oxygen diffusion. In the present study, we used a hollow-fiber bioreactor system to supply nutrients and oxygen for the cultivation of high cell-density skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique. C2C12 cells, magnetically-labeled with magnetite cationic liposomes (MCLs), were mixed with a type I collagen solution and seeded into the cell culture space of the hollow-fiber bioreactor. A magnet was then placed underneath the bioreactor to accumulate MCL-labeled cells in the space between the hollow fibers by magnetic force. Perfusion culture was performed using a myogenic differentiation medium for 7 d. Histological observation revealed that high cell-dense and viable tissue constructs containing myotubes were successfully formed. Furthermore, muscle-specific proteins, such as myosin heavy chain and tropomyosin, were detected by western blot, indicating that C2C12 cells underwent myogenic differentiation. These findings indicate that the hollow-fiber bioreactor system is an effective approach for the in vitro culture of large skeletal muscle tissue constructs, fabricated by magnetic force-based tissue engineering.
【 授权许可】
Unknown
【 预 览 】
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RO201912080696820ZK.pdf | 18KB | download |