Continuous Stereoselective Reduction Catalyzed by Thermophilic Alcohol Dehydrogenase in a Gas Phase Bioreactor

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Jochen Büchs²  Kazuhito Nagayama¹  Antje C. Spieß³ 

[1] Department of Materials Science and Engineering, Kochi National College of Technology;Biochemical Engineering, RWTH Aachen University;Enzyme Process Technology, RWTH Aachen University

关键词: Gas Phase;    Bioreactor;    Theromophilicity;    Alcohol Dehydrogenase;    Stereoselective Reduction;   

DOI  :  10.1252/jcej.11we123

来源: Maruzen Company Ltd

【 摘 要 】

References(8)Cited-By(2)The present study investigates continuous gas phase stereoselective reduction catalyzed by commercial thermophilic alcohol dehydrogenase. The alcohol dehydrogenase and cofactor β-nicotinamide adenine dinucleotide phosphate were simultaneously immobilized on non-porous spherical glass beads. The reduction of 4-methyl-2-pentanone to (S)-4-methyl-2-pentanol with concomitant cofactor regeneration by 2-propanol was employed as a model reaction. According to the differing buffer pH dependence of the enzyme activity on both substrates, the maximal reactivity was obtained at a pH of 7.5 used for immobilized enzyme preparation. Owing to the higher stereoselective production, the water activity in the feed gas phase and reaction temperature were optimized at 0.9 and 333 K, respectively.

【 授权许可】

Unknown