Production of High Concentration Glucose-1-Phosphate by Immobilized Phosphorylase

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Masaru Sakata²  Takashi Kawai¹  Hiroshi Ooshima²  Shigeto Kayane³ 

[1] Processing Development Research Laboratories, Kao Corp.;Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University;Research & Development Division, Kao Corp.

关键词: Phosphorylase;    Glucose-1-Phosphate;    G-1-P;    Immobilized Phosphorylase;    Column Reactor;   

DOI  :  10.1252/jcej.08we226

来源: Maruzen Company Ltd

【 摘 要 】

References(18)The continuous production of Glucose-1-phosphate (G-1-P) using immobilized potato phosphorylase was investigated. Potato phosphorylase was immobilized on a strongly basic anion-exchange resin, HPA-25 through contact of the resin with potato juice or wastewater of a potato-starch factory. The immobilization was highly selective for phosphorylase among proteins contained in potato juice and the wastewater. The immobilized phosphorylase could be used for the continuous G-1-P production over one year without significant deactivation. By using a 100-L column reactor packed with immobilized phosphorylase of 14.1 U·mL-carrier–1 at the superficial linear velocity of 0.034 m·h–1 and the space velocity of 3.1 × 10–9 m3·U–1·h–1, 185 mM G-1-P, which is the maximum concentration potentially expected from 516 mM dextrin and 2000 mM phosphate, was continuously produced.

【 授权许可】

Unknown