JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | |
Production of High Concentration Glucose-1-Phosphate by Immobilized Phosphorylase | |
Masaru Sakata2  Takashi Kawai1  Hiroshi Ooshima2  Shigeto Kayane3  | |
[1] Processing Development Research Laboratories, Kao Corp.;Department of Applied Chemistry and Bioengineering, Graduate School of Engineering, Osaka City University;Research & Development Division, Kao Corp. | |
关键词: Phosphorylase; Glucose-1-Phosphate; G-1-P; Immobilized Phosphorylase; Column Reactor; | |
DOI : 10.1252/jcej.08we226 | |
来源: Maruzen Company Ltd | |
【 摘 要 】
References(18)The continuous production of Glucose-1-phosphate (G-1-P) using immobilized potato phosphorylase was investigated. Potato phosphorylase was immobilized on a strongly basic anion-exchange resin, HPA-25 through contact of the resin with potato juice or wastewater of a potato-starch factory. The immobilization was highly selective for phosphorylase among proteins contained in potato juice and the wastewater. The immobilized phosphorylase could be used for the continuous G-1-P production over one year without significant deactivation. By using a 100-L column reactor packed with immobilized phosphorylase of 14.1 U·mL-carrier–1 at the superficial linear velocity of 0.034 m·h–1 and the space velocity of 3.1 × 10–9 m3·U–1·h–1, 185 mM G-1-P, which is the maximum concentration potentially expected from 516 mM dextrin and 2000 mM phosphate, was continuously produced.
【 授权许可】
Unknown
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RO201912080696356ZK.pdf | 19KB | download |