Micro-Plate Based Monolithic Ion-Exchange Chromatography for High Throughput Protein Purification Process Design

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Yuko Nishijima¹  Mitsuyo Abe¹  Sachie Fujii¹  Noriko Yoshimoto¹  Parvin Akbarzadehlaleh¹  Shuichi Yamamoto¹ 

[1] Laboratory of Bioprocess Engineering, School of Engineering and Graduate School of Medicine, Yamaguchi University

关键词: High Throughput System;    Monolithic Chromatography;    Ion-Exchange Chromatography;    96-Well Micro-Plate;    Linear Gradient Elution;   

DOI  :  10.1252/jcej.07WE184

来源: Maruzen Company Ltd

【 摘 要 】

References(22)Cited-By(1)As a method for surveying efficient separation conditions for the mobile phase, a 96-well micro-plate based monolithic ion-exchange chromatography system was developed. Discontinuous flow was accomplished by a vacuum manifold. Linear salt gradient elution experiments were carried out with this system and the elution curves were compared with those for monolithic disk column IEC. The normalized gradient slope–the peak salt concentration data for the micro-plate system agreed with those for the monolithic disk column IEC. This system is useful as the liquid volume needed for the experiment is highly reduced, and large amounts of data are obtained quickly.

【 授权许可】

Unknown