Purification and Characterization of N-Acetyl-D-Glucosamine Transferase from Pupae of Papilio xuthus Linné

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Ryosuke Maeda¹  Michiaki Matsumoto¹  Kazuo Kondo¹  Akihiro Nozawa¹ 

[1] Department of Chemical Engineering and Materials Science, Doshisha University

关键词: N-Acetyl-D-Glucosamine Transferase;    Purification;    Chitooligosaccharide;   

DOI  :  10.1252/jcej.38.455

来源: Maruzen Company Ltd

【 摘 要 】

References(5)In this work three kinds of N-acetyl-D-glucosamine transferase were purified from the crude extract of pupae of Papilio xuthus Linné through Sephadex G-25 gel filtration chromatography and CM-Sephadex C-50 ion exchange chromatography. Thermal stability of these enzymes were 35–55°C and optimal pH for transferase activity appeared at 6.5–7.0. Each enzyme could react with N-acetyl-D-glucosamine, and produced insoluble deposit suggesting highly polymerized chitooligosaccharides. These catalytic properties somewhat differ from those of chitintransferase purified from pupae of Pieridae belonging to the same order.

【 授权许可】

Unknown