Clarification of Enhanced Hydroxyl Radical Production in Fenton Reaction with ATP/ADP Based on Luminol Chemiluminescence

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

SATOKA AOYAGI²  KIYOTAKA SAKAI²  MAKOTO YAMAZAKI²  TAKEHIRO MIYASAKA¹ 

[1] Department of Physiology, Kawasaki Medical School;Department of Chemical Engineering, Waseda University

关键词: ATP;    ADP;    Luminol Chemiluminescence;    Fenton Reaction;    Hydroxyl Radical;   

DOI  :  10.1252/jcej.34.956

来源: Maruzen Company Ltd

【 摘 要 】

References(14)Cited-By(5)Enhanced hydroxyl radical production in the Fenton reaction with ATP or ADP based on luminol chemiluminescence was clarified. Luminol chemiluminescence intensity increases with the concentration of ATP or ADP due to their influence on the Fenton reagents which produce hydroxyl radicals. Fenton reagents containing hydrogen peroxide and ferrous ion produce hydroxyl radicals which attack luminol and this emits light. The effect of ATP and ADP on the Fenton reaction is detected as luminol CL intensity with a photon counting system. This method allows measurement of ATP or ADP concentration ranging from 0 to 15 mM, which covers the physiological concentration range.

【 授权许可】

Unknown