Modification of Peroxidase with Telomers Containing Amino End-Group and Their Catalytic Activity

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

TOHRU HIRAI¹  SHIMIO SATO¹  TADAHIRO MURAKATA(AITA)¹  TAKAHIRO SHIMOJIMA¹  TAKESHI HIGUCHI¹ 

[1] Department of Materials Science and Engineering, Yamagata University

关键词: Peroxidase;    Modification;    Telomer;    Animo End-Group;    Organic Media;   

DOI  :  10.1252/jcej.34.706

来源: Maruzen Company Ltd

【 摘 要 】

References(13)Two telomers containing amino end-group are synthesized and attached covalently to peroxidase by using coupling agents. When the enzyme is modified with a telomer having polystyrene as a main chain, it dissolves in organic solvents and exhibits catalytic activity in water-immiscible solvent. The catalytic activity increases as the polarity of the solvents decreases. The enzyme modified with a telomer having poly-N-isopropylacrylamide chain varies its solubility drastically from water-soluble to water-insoluble at a certain temperature, being accompanied by change in the catalytic activity. The solubility variation is reversible against temperature change and allows the catalytic activity to be controlled reversibly.

【 授权许可】

Unknown