Conservation of Root Regeneration Potential of Cell Aggregates from Horseradish Hairy Roots Used as Artificial Seeds

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Setsuji Tone¹  Vez Pagara Repunte¹  Masahito Taya¹ 

[1] Department of Chemical Engineering, Faculty of Engineering Science, Osaka University

关键词: Biochemical Engineering;    Plant Cell Culture;    Artificial Seeds;    Paraffin Coat;    Horseradish Hairy Roots;    Plant Cell Storage;   

DOI  :  10.1252/jcej.29.874

来源: Maruzen Company Ltd

【 摘 要 】

References(25)Cited-By(3)The effects of water content in agar gel used as a medium and oxygen level in the gas phase on the adventitious root regeneration of cell aggregates (CA) derived from horseradish hairy roots were investigated in cultures at 25°C, The number of roots emerging from CA was highly dependent on water content of the agar gel and no root regeneration was observed at a gel water content of 66% during culture time of 20 days. CA root regeneration was suppressed when the CA were kept for 20 days in an atmosphere oxygen composition of 10%, but was restored upon transfer of the CA to normal atmosphere of 21% oxygen. On the basis of these findings, an artificial seed was proposed using the CA as a cell inclusion material encapsulated in alginate gels covered with coats. From the perspective of conserving the root regeneration potential of the CA by preventing the drying of alginate gel while keeping oxygen availability to the CA, different coating materials of ethylene vinyl acetate acrylic acid terpolymer, paraffin and polyorganosiloxine were tested. Paraffin was selected as a suitable coating material because of its efficient drying tolerance and adequate permeability to oxygen. A regeneration efficiency of 90% could be obtained from the CA, stored in alginate gel covered with a paraffin coating of 0.40 mm thickness at 25°C for 60 days in air, when sucrose concentration in the gel was over 240 mol m–3.

【 授权许可】

Unknown