Analysis of Separation Performance for High Performance Liquid Chromatography

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

M. Ishida¹  T. Hatanaka¹ 

[1] Research Laboratory of Resources Utilization, Tokyo Institute of Technology

关键词: Liquid Chromatography;    Dimensionless Analysis;    Langmuir Isotherm;    Overloading Condition;    Operating Condition;   

DOI  :  10.1252/jcej.29.336

来源: Maruzen Company Ltd

【 摘 要 】

References(15)A diagram to systematically represent the separation performance for a wide range of operating conditions of high performance liquid chromatography is proposed. It is called a separation performance diagram, showing the relationship between the dimensionless parameter related mainly to the material, kp′, and the dimensionless parameter on the elution time, uT/εL – 1, for the specified values of yield. The parameter, kp′, is the capacity factor measured from the center of a rectangle input. It is equal to the value of uT/εL – 1 when the yield of the product is 0.5. This parameter becomes k0′ for the linear Isotherm. The line for the specified yield in the diagram becomes straight when the parameters related to non-linearity, bC0 and utp/εL, are specified. The purity of the product is determined from these yields, and the operating conditions such as time interval of sample injection tp and the cut time T can be obtained from the diagram. Since kp′ is equal to uT/εL – 1 for yield = 0.5, the value of the abscissa kp′ is determined from the location at yield = 0.5 of the elution curve obtained from experiments, and the diagram can be drawn. The effectiveness of the diagram is confirmed by applying it to obtain the period between successive injections and the cut time on two-step chromatography. It is also shown that this diagram can be applied to the separation of the mixture with interaction among components.

【 授权许可】

Unknown