Overproduction of Foreign Gene Product in Recombinant Escherichia coli by in situ Adsorption of Tryptophan

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Yoshiki Sano²  Osamu Ariga²  Hiroyuki Honda¹  Koji Ogiso¹  Takeshi Kobayashi¹  Tateki Yamakawa² 

[1] Department of Biotechnology, Faculty of Engineering, Nagoya University;Department of Fine Material Engineering, Faculty of Textile Science and Technology, Shinshu University

关键词: Biochemical Engineering;    Recombinant Escherichia coli;    Trp Promoter;    Tryptophan;    Fed-Batch Culture;    Adsorption;   

DOI  :  10.1252/jcej.27.627

来源: Maruzen Company Ltd

【 摘 要 】

References(14)Cited-By(2)For two stage culture with control of tryptophan concentration, rapid in situ removal of tryptophan by adding adsorbent was investigated. Activated carbon, BAC, could adsorb a larger amount of tryptophan than other adsorbents. From test tube cultures, it was found that 20 g/1 of BAC was necessary to remove large portions of tryptophan in a medium containing 20 mg/1 of tryptophan and induce the β-galactosidase gene expression. Two stage culture was performed using a jar fermentor in which tryptophan concentration was kept at 20 mg/l in the first stage for cell growth and then almost totally removed by direct addition of 20 g/l of BAC to the medium. One hour after addition of BAC, an increase in enzyme activity was observed. Maximum specific enzyme activity reached a final value of 320 μkat/g-protein, which corresponded to a level 12 times that of the control culture.

【 授权许可】

Unknown