Application of Pluronic F68 for Aqueous Two-Phase Extraction of Proteins

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Masamichi Kamihira¹  Hiroshi Takeuchi¹  Toshihiko Kitahara¹ 

[1] Department of Chemical Engineering, Faculty of Engineering, Nagoya University

关键词: Biochemical Engineering;    Aqueous Two-Phase Extraction;    Bioseparation;    Pluronic F68;    Binodial Curve;    Monoclonal Antibody;   

DOI  :  10.1252/jcej.26.183

来源: Maruzen Company Ltd

【 摘 要 】

References(16)Cited-By(7)Studies were made of the application of a block copolymer of polyethylene oxide and polypropylene oxide, Pluronic F68, to aqueous two-phase extraction of proteins. For Pluronic F68/salt systems, the effects of temperature, salt concentration and pH on the binodials and protein partitioning were studied. Pluronic F68/salt system had a region in which Pluronic F68 becomes solid in phase diagram at 25°C. Partitioning of the proteins between the two phases could be controlled by varying salt composition. Further applicability of the Pluronic F68/ammonium sulfate system was examined for the purification of monoclonal antibodies from various hybridoma culture broths. Aqueous two-phase extraction provided a simple and rapid separation and concentration method with high recovery.

【 授权许可】

Unknown