APPLICABILITY OF THE PSEUDO-STEADY STATE ASSUMPTION TO THE IRREVERSIBLE MICHAELIS-MENTEN REACTION

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

KATSUHIKO OKADA¹  HARUO HIKITA¹  HARUO ISHIKAWA¹ 

[1] Department of Chemical Engineering, University of Osaka Prefecture

关键词: Biochemical Engineering;    Enzyme Reaction;    Michaelis-Menten Mechanism;    Pseudo-Steady State;    Steady State Assumption;   

DOI  :  10.1252/jcej.21.267

来源: Maruzen Company Ltd

【 摘 要 】

References(13)The applicability of the pseudo-steady state assumption to an enzyme-substrate complex was examined for an irreversible single-substrate reaction which obeys the Michaelis-Menten mechanism by using computer simulation. The pseudo-steady state solutions of the time courses of the substrate and product were compared with the exact solutions calculated by using a complete set of rate equations under various conditions. The maximum relative errors of the pseudo-steady state solutions to the exact solutions could be correlated well only in terms of a dimensionless parameter, which the authors designate ψM and define by the ratio of the pseudo-steady state solution of the intermediate complex at t = 0 to the initial substrate concentration. The simulations showed that if ψM ?? 0.05, the pseudo-steady state solutions are accurate within a tolerable error of 5 % and therefore the pseudo-state assumption holds.

【 授权许可】

Unknown