INDUSTRIAL PRODUCTION OF L-ALANINE FROM AMMONIUM FUMARATE USING IMMOBILIZED MICROBIAL CELLS OF TWO KINDS

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

TETSUYA TOSA¹  ICHIRO CHIBATA¹  SATORU TAKAMATSU¹ 

[1] Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.

关键词: Biochemical Engineering;    Immobilized Microbial Cell;    L-Alanine Production;    Sequential Enzyme Reaction;    Immobilized Escherichia coli;    Immobilized Pseudomonas dacunhae;   

DOI  :  10.1252/jcej.19.31

来源: Maruzen Company Ltd

【 摘 要 】

References(14)Cited-By(7)Rate equations were derived for a two-step enzyme reaction which produced L-alanine from ammonium fumarate via L-aspartic acid by aspartase of immobilized Escherichia coli cells and L-aspartate β-decarboxylase of immobilized Pseudomonas dacunhae cells. To establish an efficient system for industrial production of L-alanine, we investigated the design of adequate bioreactors on the basis of the simulation of this sequential reaction by solving the simultaneous equations derived. As a result, L-alanine was found to be produced efficiently by use of two sequential column reactors: a conventional column reactor containing immobilized E. coli cells and a closed column reactor containing immobilized P. dacunhae cells.

【 授权许可】

Unknown