PRODUCTION OF L-ALANINE FROM AMMONIUM FUMARATE USING TWO MICROBIAL CELLS IMMOBILIZED WITH k-CARRAGEENAN

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

TETSUYA TOSA¹  ICHIRO CHIBATA¹  SATORU TAKAMATSU¹ 

[1] Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd.

关键词: Biochemical Engineering;    Immobilized Microbial Cell;    L-Alanine Production;    Two Step Reaction;    Immobilized Escherichia coli;    Immobilized Pseudomonas dacunhae;   

DOI  :  10.1252/jcej.18.66

来源: Maruzen Company Ltd

【 摘 要 】

References(15)Cited-By(6)To produce L-alanine efficiently with immobilized microbial cells, the production system from ammonium fumarate by two-step enzyme reaction using aspartase activity of Escherichia coli cells and L-aspartate β-decarboxylase activity of Pseudomonas dacunhae cells in a single reactor was examined. It was found that the reaction should be started at pH around 8 and that the use of a mixture of immobilized E. coli cells and immobilized P. dacunhae cells was superior to that of co-immobilized E. coli-P. dacunhae cells.

【 授权许可】

Unknown