Pilot Study on the Stability ofInternal Quality Control SamplesFor Anti-HCV Immunodiagnostic Assays

International Journal of Biomedical and Advance Research

Pankaj Kumar Sharma¹  Charu Mehra Kamal¹  Reba Chhabra¹  Aakanksha Yadav¹  Brij Bhushan¹  Sanjeev Kumar Sharma¹  Rajeev Kumar¹ 

关键词: Hepatitis C;    HCV;    Antibodies;    Stability;    Internal Quality Control;    IQC;   

DOI  :  10.7439/ijbar.v6i12.2828

学科分类：药学、药理学、毒理学（综合）

来源: Scholar Science Journals

【 摘 要 】

Laboratory diagnosis of HCV infection is usually made on the basis of the detection of circulating antibodies. All laboratories carrying out anti-HCV tests, should have a well-functioning quality assurance programme. Quality control (QC) materials are crucial for Internal Quality Control (IQC) and External Quality Assessment Scheme (EQAS). Therefore, preparing IQC samples is cost effective QC material. The present study was carried out to identify the effects of temperature on stability of anti-HCV antibodies in plasma during storage by evaluating anti-HCV antibodies using sensitive and specific ELISA assays intended for detection of anti-HCV antibodies. The stability of anti-HCV positive samples was assessed at three different temperatures 37??, 48?? and 65?? incubated for a period of 7 days and 14 days by two ELISA Kits. A significant decrease in E-ratios when stored at higher temperatures was observed in the present study.?

【 授权许可】

CC BY