Paraoxonase1 activity, its Q192R polymorphism and diabetic retinopathy in type 2 diabetes mellitus.

International Journal of Biomedical and Advance Research

Mahesh Harishchandra Hampe¹  Mukund Ramchandra Mogarekar¹ 

关键词: paraoxonase1;    polymorphism;    diabetic retinopathy;    diabetes mellitus.;   

DOI  :  10.7439/ijbar.v5i1.571

学科分类：药学、药理学、毒理学（综合）

来源: Scholar Science Journals

【 摘 要 】

ABSTRACT?Background : The goal of this study was to investigate the role of paraoxonase1 (PON1) status i.e. activity and Q192R polymorphism, in the development of diabetic retinopathy (DR) in patients of type 2 diabetes mellitus.?????????????????????????????????????????????????????????????????????? ? ?????????????????????????????????????????? Methods : This cross-sectional study included 55 normotensive type 2 diabetic patients (duration more than 5 years) admitted in the hospital divided into two groups (with and without DR) on the basis of fundus examination by direct ophthalmoscopy. Serum samples of all patients were subjected for PON1 activity with and without salt using paraoxon as substrate and arylesterase activity of PON1 using phenylacetate as substrate. PON1 phenotyping is carried out using ratio of salt stimulated PON1 activity to arylesterase activity. Results : Arylesterase activity of PON1 was found significantly lower in diabetics with DR than diabetic patients without DR ( P =0.01). Multivariate testing also showed its independent protective association in the development of DR (OR 0.958, [95% CI (0.915-1.004)],Pvalue = 0.023). Moreover, DR was associated significantly in diabetic patients with PON1 R allele carriers (71.05%) than diabetic patients with PON1 QQ homozygotes (29.41%). PON1 R carriers had higher Odds ratio (OR) for DR in univariate analysis (OR 5.891, [95% CI (1.676-20.705)],Pvalue = 0.006) and in multivariate analysis (OR 14.12 [95% CI (0.926-215.2)],P =0.057) after adjustment of conventional risk factors of diabetes. Also diabetics with homozygous PON1 RR were associated significantly with severe forms of DR. Conclusions : Arylesterase activity of PON1 may be more important in the risk of retinopathy in diabetic individual than the paraoxon hydrolytic activity. Moreover PON1 R allele is associated with susceptibility to DR showing a graded risk relationship to the number ofRalleles. Thus the determination of PON1 status may be useful in the risk assessment and management of DR.?

【 授权许可】

CC BY