An in-vitro investigation of the antioxidant activity of Phyllanthus amarus

International Journal of Biomedical and Advance Research

A. V. Chandewar¹  H. J. Dhongade¹ 

关键词: Phyllanthus amarus;    total phenolic content;    antioxidant;    1;    1-diphenyl-2-picrylhydrazyl radical;    β–carotene-linoleate method;   

DOI  :  10.7439/ijbar.v4i6.418

学科分类：药学、药理学、毒理学（综合）

来源: Scholar Science Journals

【 摘 要 】

The aim of this work was to investigate the total phenolic content and antioxidant potential of aqueous extract ofPhyllanthus amarus . The aqueous extract of?Phyllanthus amaruswhole plant was prepared then systematically investigated by various antioxidant assays, including: DPPH- (1, 1-diphenyl-2-picrylhydrazyl radical), Nitric oxide radical inhibition activity, and ???arotene-linoleate method, compared with ascorbic acid and rutin. Its total phenolic content was also measured by Folin-Ciocalteu colorimetric method.P. amarusaqueous extract exhibited effective antioxidant activity in dose-dependent manners and its IC 50values were calculated as 4.21?0.379, 426?0.512, 126?0.348 for DPPH, Nitric oxide radical inhibition activity, and ???arotene-linoleate assay respectively. Its total phenolic content was 52.71? 0.214 mg GAE /g.P. amarusaqueous extract has effectivein vitroantioxidant activity which may attribute to its total phenolics, among which phyllanthin and hypophyllanthin can be considered as the active components. The pharmacological effects or healthcare functions ofP. amarusmay result from the synergistic effects caused by the combination of its components and its antioxidant effect plays an important role in the synergistic effects.

【 授权许可】

CC BY