Comparative Analysis of mpb-64 and Nested PCR targeting IS6110 for the Detection of Extra Pulmonary Tuberculosis

International Journal of Biomedical and Advance Research

Satish C Nautiyal¹  Mukesh Kumar¹  R. K. Singh¹  Natasha K Ahmed¹  Rakhi Kaushik¹  Shivani Sailwal¹  Narotam Sharma¹ 

关键词: Extra-pulmonary samples;    Copy Number;    Molecular targets;    Amplimers;    Lymph node;    Polymerase Chain Reaction;   

DOI  :  10.7439/ijbar.v4i10.511

学科分类：药学、药理学、毒理学（综合）

来源: Scholar Science Journals

【 摘 要 】

Introduction : Extra Pulmonary Tuberculosis can be seen in more than half of the patients with concurrent AIDS and tuberculosis. Methodology:A total of ninety clinical specimens, which includes; 14 pus, 32 CSF, 4 ascetic fluid, 6 biopsy, 10 blood, 3 urine, 6 endometrial tissues, 6 pleural fluids, 1 endometrial curetting, and 2 synovial fluid, collected from the various departments of SMI Hospital, Patel Nagar, Dehradun. Specimens were further processed for ZN staining, PCR utilizing; mpb-64 and nested PCR (N-PCR) using IS6110 as a target for the detection of extra pulmonary tuberculosis cases. Results:Out of 90 clinical specimens processed, 29 (32.2%) came N-PCR positive and 23 (25.5%) arempb64TB PCR positive. When analyzed comparatively for both the molecular targets, it was seen that 20 samples came positive by both the targets i.e.IS6110as well asmpb64gene, but 9 specimens alone came positive only byIS6110NTPCR, and the same came negative formpb64gene. 3 specimens showed positive result status formpb64gene by conventional assay and at the same time they were negative forIS6110gene. Conclusion:An essential element in the management of extra pulmonary tuberculosis is the availability of rapid, sensitive and specific identification of the causative agent. The laboratory diagnosis is largely based on direct microscopy and culture for mycobacterium. Direct microscopic examination is used to detect acid-fast bacilli (AFB) & has very low sensitivity. The gold standard for isolation of mycobacterium is not only time-consuming but also has low sensitivity in case of extra pulmonary tuberculosis. TB PCR diagnostics kits must be prepared, targeting mpb-64 as well as IS6110 as a targets to avoid false results.?

【 授权许可】

CC BY