DYNAMIC IMAGING OF INTRACELLULAR CA2+ CONCENTRATION IN INSULIN SECRETING MIN6 Î’-CELLS USING WIDE FIELD AND TIRF MICROSCOPY

International Journal of Biomedical and Advance Research

Vinita Pawar¹  Archana Tiwari¹  Priyanka Sharma¹  Shiv Kumar Mishra¹ 

关键词: β-cells;    Dynamics;    Glucose;    Imaging;    Insulin and Insulinoma;   

DOI  :  10.7439/ijbar.v3i10.327

学科分类：药学、药理学、毒理学（综合）

来源: Scholar Science Journals

【 摘 要 】

Since 1920's major medical advance based on animal research i.e. Insulin for diabetes. Diabetes is world????s most common metabolic disorder characterised by pancreatic ??-cells dysfunction. Normally these cells respond to high glucose concentrations following exocytosis of stored insulin trigger by the combined action of store-operated calcium entry (SOCE) and calcium-release activated current (CRAC) channel, with an elusive mechanism. To illustrate it, dynamic imaging may used as power full tool as, it offers power of capturing the dynamics of biological action in live cells. In this review we focuses on dynamic imaging of pancreatic MIN6 cells, an appropriate??model used as an alternative to animal experiments in diabetes research to understand what is occurring, in and among pancreatic ??-cells and influence their behaviour in desired ways, using advanced wide field and total internal reflection fluorescence microscopy (TIRFM). This may empower the principles of optimization of diabetes research. "

【 授权许可】

CC BY