Infertility and Male Mating Behavior Deficits Associated With Pde1c in Drosophila melanogaster

Genetics

Dennis J. Hazelett¹  Anke Vermehren-Schmaedick¹  David B. Morton¹  Rachel Clemens-Grisham¹ 

DOI  :  10.1534/genetics.110.118018

学科分类：医学（综合）

来源: Genetics Society of America

【 摘 要 】

Pde1c is a calcium/calmodulin-regulated, dual-specificity cyclic nucleotide phosphodiesterase. We have used a transposon insertion line to investigate the physiological function of Pde1c in Drosophila melanogaster and to show that the insertion leads to male sterility and male mating behavior defects that include reduced copulation rates. Sterility appears to be primarily due to elimination of sperm from the female reproductive system. The male mating behavior defects were fully rescued by expression of exogenous Pde1c under the control of either a Pde1c or a pan-neuronal promoter, whereas the sterility could be only partially rescued by expression of exogenous Pde1c under the control of these promoters. We also show that Pde1c has a male-specific expression pattern in the CNS with an increased number of Pde1c-expressing neurons in the abdominal ganglion in males.

【 授权许可】

Unknown