RT-PCR cytokine study in patients with allergic rhinitis

Revista Brasileira de Otorrinolaringologia

Nascimento, Evaldo¹  Silva, Tarcimara Moreira da¹  USP¹  Araújo, Ricardo Nascimento¹  Guimarães, Roberto Eustáquio Santos¹  Nunes, Flávio Barbosa¹  UFMG¹  FEAD Centro de Gestão Empreendedora¹  Becker, Helena Maria Gonçalves¹ 

关键词: allergen;    cytokines/immunology;    hypersensitivity;    rhinitis/allergic/perennial;    rhinitis/allergic/seasonal;    skin/tests.;   

DOI  :  10.1590/S0034-72992009000100004

学科分类：医学（综合）

来源: Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervicofacial

【 摘 要 】

Allergic rhinitis is an inflammatory reaction of the nasal mucosa, in consequence of an IgE mediated hypersensitive reaction to inhaling allergens, involving different mediators and cytokine cells.AIM: The purpose of this study was to evaluate the transcriptions for IL-4, IL-5, IL-8 and IFN-gama, particularly important in the nasal allergy process, especially IL-4 and IL-5. For this study we decided to evaluate atopic patients who were free from allergic crises, with the purpose of knowing the cytokine expressions during this period.MATERIALS AND METHODS: Another prospective and transversal study was carried out, selecting 30 patients, 13 of these patients were pauci-symptomatic and 17 were non atopic. The groups were selected by means of a medical interview, an otolaryngologic clinical exam and allergy skin tests - Prick Test. The cytokines were investigated in fragments of the nasal mucosa, using RT-PCR - chosen because it has good reproducibility and specificity.RESULTS: IL-5, IL-8, IFN-gama cytokine values were kept homogeneous in relation to the control group. Only IL-4 presented significant statistic differences.CONCLUSION: Asymptomatic patients with allergic rhinitis presented with normalization of cytokine expression in the nasal mucosa, with exception of IL-4.

【 授权许可】

Unknown