Effect of mitomicin C in eosinofilic nasal polyps estromal culture: induction of apoptosis in eosinophil

Revista Brasileira de Otorrinolaringologia

Nascimento, Evaldo¹  Crosara, Paulo Fernando Tormin Borges¹  Universidade Federal de Minas Gerais¹  Sobrinho, Sebastião C.¹  Nunes, Flávio B.¹  Becker, Helena M.G.¹  Calosimo, Enrico A.¹  Vasconcelos, Anilton C.¹  Guimarães, Roberto E. S.¹ 

关键词: nasal polyps;    eosinophils;    mitomycin C;    apoptosis.;   

DOI  :  10.1590/S0034-72992004000500013

学科分类：医学（综合）

来源: Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervicofacial

【 摘 要 】

The eosinophilic nasal polyposis occurs as a manifestation of several diseases. It affects multiple nasal sinuses and presents with high relapsing rates after current treatment. The eosinophils play an essencial role in the pathogenesis through their low apoptosis rates and the long periods of remaining in the tissues in an active form.AIM: During the present study the effect of C-mitomycin was observed in the apoptosis induction of stromal eosinophils in eosinophilic nasal polyps.STUDY DESIGN: Case control.MATERIALS AND METHOD: During this auto-matched study, 9 samples of eosinophilic nasal polyps were cultivated in RPMI 1640 medium and then evaluated at the beginning, and after 12 and 24 hours of incubation. The study group was submitted to C-mitomycin in a 400 µg/ml concentration during 5 minutes. Both control and study group cultures were evaluated histopathologically and their apoptotic rates were determined. They were stained using hematoxilin-eosin and observed through microscopic enhancement of 1000 times.RESULTS: The analysis of 674 digitalized fields showed that those cultures treated with C-mitomycin achieved significantly higher apoptotic rate after 12 hours of incubation when compared to control group rate (p<0,001).CONCLUSION: This study supports that C-mitomycin is efficacious in the induction of the apoptosis of stromal eosinophils in eosinophilic nasal polyps.

【 授权许可】

Unknown