Leaf Surface Bacterial Colonization in Response to Brevicoryne brassicae (L.) Infestation of Brassica chinensis L. | Science Publications

American Journal of Microbiology

S. Raychaudhuri¹  I. Mewis¹  C. Ulrichs¹ 

关键词: Epiphytic microbial;    Brassica chinensis;    aphid infested group;    Brevicoryne brassicae;    phyllospheric bacterial;    Genomic DNA;    scanning electron microscope;    statistical analysis;   

DOI  :  10.3844/ajmsp.2010.36.41

学科分类：微生物学和免疫学

来源: Science Publications

【 摘 要 】

Problem statement: Epiphytic population is an intrinsic part of the leaf surface. The effectof stress like insect infestation is not well understood. The aim of the study was to determine possibleeffects of insect infestation on bacteria community. Approach: The leaf surface microbial profile ofBrassica rapa ssp. chinensis (L.) was investigated using a culture-dependent approach under normaland infested condition. Results: The presence of mostly novel Gram-negative bacilli on the leafsurface of B. chinensis was evident, with maximum identity with members of the phyla Firmicutes andActinobacteria at the 16S rDNA level, as evident from GenBank accession numbers FJ231349 toFJ231355. The specialist aphid Brevicoryne brassicae (L.) was used as a model to study the effect ofinsect infestation on the epiphytic leaf surface microbial populations in B. chinensis. Plants infectedwith aphids had significantly higher bacteria counts than non-infested plants. Conclusion: Thesedifferences were up to 10,000-fold within three days post infestation. This increase is probably due tothe secretion of honeydew, which acts as an additional carbon source for the pre-existing microbes.However, aphid infestation had no effect on the qualitative variation of microbes on the plants, whichrules out the abundance of microbial population on the leaf as a contribution of the aphids alone.

【 授权许可】

Unknown