Improved method to obtain pfaffic acid as a marker for quality control

Química Nova

Rodrigues, Marili Villa Nova¹  Montanari Junior, Ilio¹  Universidade Estadual de Campinas, Campinas, Brasil¹  Rehder, Vera Lucia Garcia¹  Rodrigues, Rodney Alexandre Ferreira¹  Vedovello, Amanda¹ 

关键词: pfaffic acid;    Hebanthe eriantha;    Pfaffia paniculata.;   

DOI  :  10.1590/S0100-40422013000500019

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Pfaffic acid, a marker of Hebanthe eriantha (Brazilian ginseng), was first isolated in 1983 but is not yet commercially available. This lack of availability compromises the quality control of this plant and its derivatives. This paper proposes a process for pfaffic acid isolation from roots of H. eriantha at a purity suitable for analytical purposes. The steps involved in this process included extraction, hydrolysis, fractionation and purification by preparative HPLC. This process led to isolation of pfaffic acid with a chromatographic purity of 98.5% in a 0.25% yield from dried roots of H. eriantha; this yield is more than forty times higher than that of the current method in the literature.

【 授权许可】

Unknown