Copper fractionation in proteins from plasma, muscle and liver of Nile tilapia

Química Nova

Lima, Paula M.¹  Moraes, Paula M.¹  Universidade Estadual Paulista, Botucatu, Brasil¹  Universidade Estadual Paulista, Campinas, Brasil¹  Neves, Renato C. F.¹  Castro, Gustavo R.¹  Padilha, Pedro M.¹  Santos, Felipe A.¹  Baldassini, Welder A.¹ 

关键词: Nile tilapia;    metalloproteins;    metallomics.;   

DOI  :  10.1590/S0100-40422012000300010

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Copper fractionation in plasma, muscle and liver of Nile tilapia was performed after protein separation by 2D-PAGE. SR XRF analysis indicated the presence of copper in three protein spots of plasma, and in two protein spots of muscle and liver, respectively. Copper ions were found to be distributed mostly in proteins that had a molar mass of less than 54 kDa and greater than 13 kDa and a pI in the 5.3-9.3 range. The copper concentration bound to these proteins was determined by GFAAS which showed concentrations in the 1.20-4.82 mg g-1 range.

【 授权许可】

Unknown