Serum quantification of vitamin C by HPLC-UV and stability study

Química Nova

Freitas, Fernando de¹  Brucker, Natália¹  Roehrs, Miguel¹  Garcia, Solange Cristina¹  Bulcão, Rachel¹  Baierle, Marília¹  Charão, Mariele¹  Durgante, Juliano¹  Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil¹  Bairros, André de¹  Moreira, Ana Paula¹ 

关键词: HPLC;    vitamin C;    stability.;   

DOI  :  10.1590/S0100-40422012000200030

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Vitamin C, an exogenous antioxidant, is essential to human health. In this study, a method was validated to serum vitamin C quantification by HPLC-UV. Its stability with and without the use of tris [2-carboxy-ethyl] phosphine hydrochloride (TCEP), at -20 and -80 °C, in serum and supernatant were also evaluated. Analysis showed r2 > 0.99, precision CV% < 15% and % bias < 15%, being linear, precise and accurate. The stability test revealed that using TCEP in serum storage at -20 and -80 °C or in supernatant at -80 °C the vitamin C levels remain stable for 30 and 12 days, respectively.

【 授权许可】

Unknown