Liquid-phase microextraction for simultaneous chromatographic analysis of three antidepressant drugs in plasma

Química Nova

Martins, Isarita¹  Siqueira, Maria Elisa Pereira Bastos de¹  Universidade de São Paulo, São Carlos, Brasil¹  Porto, Carlos Eduardo Dobrovolskni¹  Araújo, Rafhaella Carolina Cedro¹  Universidade Federal de Alfenas, Alfenas, Brasil¹  Santos-Neto, Álvaro José dos¹  Freitas, Daniela Fernanda de¹  Maia, Patrícia Penido¹ 

关键词: LPME;    antidepressants;    HPLC-FL.;   

DOI  :  10.1590/S0100-40422012000100014

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A method using Liquid Phase Microextraction for simultaneous detection of citalopram (CIT), paroxetine (PAR) and fluoxetine (FLU), using venlafaxine as internal standard, in plasma by high performance liquid chromatography with fluorescence detection was developed. The linearity was evaluated between 5.0 and 500 ng mL-1 (r > 0.99) and the limit of quantification was 2.0, 3.0 and 5.0 ng mL-1 for CIT, PAR and FLU, respectively. Therefore, it can be applied to therapeutic drug monitoring, pharmacokinetics or bioavailability studies and its advantages are that it necessary relatively inexpensive equipment and sample preparation techniques.

【 授权许可】

Unknown