Characterization of the antibiotic doripenem using physicochemical methods: chromatography, spectrophotometry, spectroscopy and thermal analysis

Química Nova

Silva, Fabiana E. B. da¹  Paula, Fávero R¹  Sayago, Carla T. M¹  Garcia, Cássia V¹  Barbosa, Fábio¹  Denardin, Elton L. G¹  Mantovani, Luciano¹  Mendez, Andreas S. L¹  Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil¹  Schapoval, Elfrides E. S¹  Universidade Federal do Pampa, Uruguaiana, Brasil¹ 

关键词: doripenem;    qualitative analysis;    physicochemical methods.;   

DOI  :  10.1590/S0100-40422011000900024

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Doripenem was characterized through physicochemical and spectroscopic techniques, as well as thermal analysis. TLC (Rf = 0.62) and HPLC (rt = 7.4 min) were found to be adequate to identify the drug. UV and infrared spectra showed similar profile between doripenem bulk and standard. The 1H and 13C NMR analysis revealed chemical shifts that allowed identifying the drug. Thermal analysis demonstrated three steps with mass loss, at 128, 178 and 276 ºC. The work was successfully applied to qualitative analysis of doripenem, showing the reported methods can be used for physicochemical characterization of doripenem.

【 授权许可】

Unknown