Optimization of saponin extraction conditions from Ampelozizyphus amazonicus by using experimental design and surface response methodology

Química Nova

Universidade Federal do Amazonas, Manaus, Brasil¹  dos Santos, Fabiane M¹  Poppi, Ronei J¹  Universidade Federal do Espírito Santo, Vitória, Brasil¹  Fundação Oswaldo Cruz, Rio de Janeiro, Brasil¹  Barthus, Rosangela C¹  Amaral, Ana C. F¹  Simões, Jersika C¹  Universidade Estadual de Campinas, Campinas, Brasil¹  Silva, Jefferson R. A¹ 

关键词: saponin;    Ampelozizyphus amazonicus;    surface response methodology.;   

DOI  :  10.1590/S0100-40422011000900023

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

This works describes the use of experimental design and surface response methodology for optimization of saponin extraction from Ampelozizyphus amazonicus. For this purpose, a method employing extraction based on maceration assisted by ultrasound technique was utilized. The following factors were studied: extraction length of time and solvent composition. The total saponin was determined by using a gravimetric method and the results expressed by their relative proportion to total crude extract. For the specific condition, 60% hydro-alcoholic solution and 18 minutes extraction length of time has shown the best results. This method can be useful for extraction of substances with biological importance.

【 授权许可】

Unknown