Solid state Differential Pulse Voltammetry (DPV) from spots of Thin Layer Chromatography (TLC): a new method for analysis of antioxidant phytoactives

Química Nova

Universidade Federal de Goiás, Goiânia, Brasil¹  Gil, Eric S.¹  Conceição, Edemilson C.¹  Reis, Nathasha S.¹  Couto, Renê O.¹  Gonçalves, Darlene¹ 

关键词: chemically modified electrodes;    rosmarinic acid;    TLC-DPV.;   

DOI  :  10.1590/S0100-40422011000200028

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

A new electroanalytical method coupling TLC-DPV in solid state was developed for quantitative determination of phytoantioxidants with medicinal purpose, e.g. rosmarinic acid (RA) in samples of phytopharmaceuticals, e.g. rosemary (Rosmarinus officinalis L.). The method showed to be feasible, presenting linearity in concentrations ranging from 0.694 x 10-3 to 9.526 x 10-3 mol L-1 (r = 0.9945), good sensibility, selectivity, reproducibility, repeatability, agility and affordable cost. The concentrations of RA in different extracts of rosemary ranged from 0.05 to 0.52 (% w/w), presenting high recovery levels when compared to HPLC.

【 授权许可】

Unknown