Method validation to hmf determination in honey by HPLC-UV and its influence on the product quality

Química Nova

Universidade Estadual do Sudoeste da Bahia, Vitória da Conquista, Brasil¹  Lemos, Gisele da Silveira¹  Santos, José Soares dos¹  Santos, Maria Lúcia Pires dos¹ 

关键词: HMF;    honey;    HPLC-UV.;   

DOI  :  10.1590/S0100-40422010000800012

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

In honey 5-hydroxymethyl-2-furaldehyde (HMF) is one of the most typical products of degradation: it is usually absent in fresh honey, but its concentration tends to rise as a result of heating processes or long-term storage. The validation protocol was performed in terms of detection and quantification limits, precision (by repeatability and intermediate precision), linearity and accuracy (by recovery tests). The method has been tested on 15 honey samples of different ages and geographical origin. HMF correlated highly with the age of the samples has been considered a very important parameter to put these honeys on the market or not and/or to estimate their shelf life.

【 授权许可】

Unknown