Ultrathin cellulose ester films: preparation, characterization and protein immobilization

Química Nova

Petri, Denise F. S.¹  Universidade de São Paulo, São Carlos, Brasil¹  Universidade de São Paulo, São Paulo, Brasil¹  Amim Jr., Jorge¹  Maia, Francisco C. B.¹  Miranda, Paulo B.¹ 

关键词: cellulose ester;    protein adsorption;    film characterization.;   

DOI  :  10.1590/S0100-40422010001000011

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

In this study cellulose acetate butyrate (CAB) and carboxymehtylcellulose acetate butyrate (CMCAB) films adsorbed onto silicon wafers were characterized by means of ellipsometry, atomic force microscopy (AFM), sum frequency generation spectroscopy (SFG) and contact angle measurements. The adsorption behavior of lysozyme (LIS) or bovine serum albumin (BSA) onto CAB and CMCAB films was investigated. The amounts of adsorbed LIS or BSA onto CMCAB films were more pronounced than those onto CAB films due to the presence of carboxymethyl group in the CMCAB structure. Besides, the adsorption of BSA molecules on CMCAB films was more favored than that of LIS molecules. Antimicrobial effect of LIS bound to CAB or CMCAB layers was evaluated using Micrococcus luteus as substrate.

【 授权许可】

Unknown