Development and validation of analytical methodology by HPLC-IR for evaluation of artemisinin on Artemisa annua L

Química Nova

Celeghini, Renata Maria dos Santos¹  Foglio, Mary Ann¹  Silva, Ana Paula da¹  Universidade Estadual de Campinas, Campinas, Brasil¹  Rodrigues, Rodney Alexandre Ferreira¹  Sousa, Ilza Maria de Oliveira¹ 

关键词: Artemisia annua L.;    clean-up;    liquid chromatographic.;   

DOI  :  10.1590/S0100-40422009000400009

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The aim of this work was to develop and validate an analytical methodology for determination of artemisinin used as antimalaric. The method was based on high performace liquid chromatography, using a CN column with mobile phase composed of methanol : H2O 50:50 (V/V). The results showed that the method presented linearity from 50 to 1500 µg/mL. It was considered selective, accurate, precise according to the specific resolution from ANVISA, the Brazilian regulatory agency.

【 授权许可】

Unknown