Development and validation of a method of analysis of glyphosate in soy grains

Química Nova

Matta, Marcia Helena de Rizzo da¹  Montagner, Émerson¹  Abreu, Adley Bergson Gonçalves de¹  Universidade Federal de Mato Grosso, Cuiabá, Brasil¹ 

关键词: glyphosate;    glyphosate-resistant soybean;    pesticide.;   

DOI  :  10.1590/S0100-40422008000100002

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The main objective of this work is to develop an efficient procedure to determine glyphosate in soybean grains. The cleanup of the aqueous extracts was done in two steps, beginning with liquid-liquid partitioning and then solid-phase extraction with anion exchange resin. After derivatization with a mixture of trifluoroacetic anhydride (TFAA) and trifluoroethanol (TFE), quantification was done by gas chromatography coupled to mass spectrometry. The mean recovery and RSD of the spiked samples were, respectively, 80.5% and 3.1% at level 0.200 mg kg-1, 93.3% and 18.7% at level 0.500 mg kg-1 and 92% and 3.5% at level 1.000 mg kg-1. The method was linear in the working range (correlation coefficient = 0.9965).

【 授权许可】

Unknown