Extraction, purification and biochemical characterization of a peroxidase from Copaifera langsdorffii leaves

Química Nova

Toyama, Marcos¹  Marangoni, Sergio¹  Pastore, Gláucia Maria¹  Smolka, Marcus¹  Universidade Federal de Alfenas, Alfenas, Brasil¹  Gouvêa, Cibele Marli Cação Paiva¹  Maciel, Hermelinda Penha Freire¹  Universidade Estadual de Campinas, Campinas, Brasil¹ 

关键词: Copaifera langsdorffii;    peroxidase;    vegetal extract.;   

DOI  :  10.1590/S0100-40422007000500003

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The aim of this work is to obtain, purify and characterize biochemically a peroxidase from Copaifera langsdorffii leaves (COP). COP was obtained by acetone precipitation followed by ion-exchange chromatography. Purification yielded 3.5% of peroxidase with the purification factor of 46.86. The COP optimum pH is 6.0 and the temperature is 35 ºC. COP was stable in the pH range of 4.5 to 9.3 and at temperatures below 50.0 ºC. The apparent Michaelis-Menten constants (Km) for guaiacol and H2O2 were 0.04 mM and 0.39 mM respectively. Enzyme turnover was 0.075 s-1 for guaiacol and 0.28 s-1 for hydrogen peroxide. Copaifera langsdorffii leaves showed to be a rich source of active peroxidase (COP) during the whole year. COP could replace HRP, the most used peroxidase, in analytical determinations and treatment of industrial effluents at low cost.

【 授权许可】

Unknown