Production, purification, cloning and application of lytic enzymes

Química Nova

Sato, Hélia Harumi¹  Fleuri, Luciana Francisco¹  Universidade Estadual de Campinas, Campinas¹ 

关键词: b-1;    3 glucanases;    lytic proteases;    chitinases.;   

DOI  :  10.1590/S0100-40422005000500026

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Lytic enzymes such as b-1,3 glucanases, proteases and chitinases are able to hydrolyse, respectively, b-1,3 glucans, mannoproteins and chitin, as well as the cell walls of many yeast species. Lytic enzymes are useful in a great variety of applications including the preparation of protoplasts; the extraction of proteins, enzymes, pigments and functional carbohydrates; pre-treatment for the mechanical rupture of cells; degradation of residual yeast cell mass for the preparation of animal feed; analysis of the yeast cell wall structure and composition; study of the yeast cell wall synthesis and the control of pathogenic fungi. This review presents the most important aspects with respect to lytic enzymes, especially their production, purification, cloning and application.

【 授权许可】

Unknown