Elucidating the Mode of Action of Marine Natural Products through an Immunoaffinity Fluorescent (IAF) Approach

Journal of the Brazilian Chemical Society

University of California, La Jolla, USA¹  Fenical, William¹  Costa-Lotufo, Leticia V.¹  Universidade de São Paulo, São Paulo, Brazil¹  La Clair, James J.¹ 

关键词:  marine natural product;    mode of action;    drug discovery;    target identification;    confocal microscopy;   

DOI  :  10.5935/0103-5053.20160148

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Understanding the target and mode of action (MOA) of small molecules activity has become a critical feature in guiding the transition between drug discovery and clinical evaluation. While classically placed at the latter stages of a discovery program, we now describe how addressing the mode of action of natural products at the early stages of a program provides an important vehicle to inform the drug discovery process. In this review, we outline a streamlined cellular and molecular biological system and explore its utility through a series of four cases studies. We believe that this approach offers critical lessons to guide future drug discovery programs.

【 授权许可】

Unknown