Chemotaxonomy of the Amazonian Unonopsis Species Based on Leaf Alkaloid Fingerprint Direct Infusion ESI-MS and Chemometric Analysis

Journal of the Brazilian Chemical Society

Pinheiro, Maria L. B.¹  Koolen, Hector H. F.¹  Silva Filho, Francinaldo A.¹  Almeida, Richardson A.¹  Souza, Afonso D. L.¹  Universidade Federal do Amazonas, Manaus, Brazil¹  Lima, Bruna R.¹  Universidade do Estado do Amazonas, Manaus, Brazil¹  Soares, Elzalina R.¹  Silva, Felipe M. A.¹ 

关键词:  alkaloid fingerprint;    aporphinoid alkaloids;    chemotaxonomy;    Unonopsis;   

DOI  :  10.5935/0103-5053.20150296

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Unonopsis (Annonaceae) is a neotropical genus constituted by nearly fifty species, with fifteen described in Brazil. In the state of Amazonas seven species are found, including U. guatterioides, which displays problems from the botanical viewpoint. Previous studies showed this genus as a promising source of aporphinoid alkaloids. In order to investigate the potential of the leaf alkaloid fingerprint for chemotaxonomic approaches, twelve Unonopsis specimens, representing five species commonly found in the state of Amazonas were subjected to acid-base partitioning to yield the respective alkaloidal fractions. These fractions were analysed by direct infusion electrospray ionization multiple stage mass spectrometry (ESI-MSn). The obtained data were treated through chemometric tools [principal components analysis (PCA) and hierarchical cluster analysis (HCA)]. Multivariate analysis pointed to aporphine, proaporphine and tetrahydroprotoberberine alkaloids as the responsible compounds for segregation of the investigated species, being these alkaloids tentatively identificated by multiple stage mass spectrometry. The alkaloid fingerprint along with multivariate analysis provided a simple and effective approach to differentiateUnonopsis species commonly found in the state of Amazonas.

【 授权许可】

Unknown