Determination of Aspirin Using Chemiluminescence System of Tris(1,10 phenanthroline)Ruthenium(II)-Cerium(IV)

Journal of the Brazilian Chemical Society

Pishkhani, Hatameh F.¹  Delouei, Nastaran J.¹  Ebrahimi, Aida¹  Keyvanfard, Mohsen¹  Golestan University, Gorgan, Iran¹  Mokhtari, Ali¹  Karimian, Hossein¹  Emami, Iraj¹  Islamic Azad University, Isfahan, Iran¹  Payame Noor University, Behshahr, Iran¹  Isfahan University of Technology, Isfahan, Iran¹ 

关键词:  chemiluminescence;    tris(1;    10 phenanthroline)ruthenium(II);    aspirin;    plasma;    tablets;   

DOI  :  10.5935/0103-5053.20150288

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

A new chemiluminescence (CL) method has been proposed for the simple determination of aspirin (acetylsalicylic acid, ASA). The method is based on the enhancement effect of ASA in the CL reaction of tris(1,10 phenanthroline)ruthenium(II), Ru(phen)32+, with cerium(IV) (CeIV) in acidic medium. Under optimum conditions, the CL intensity was proportional to the concentration of the drug in solution over the range 0.72-72.00 µg mL-1 ASA. The limit of detection (S/N = 3) was 0.45 µg mL-1. In the method proposed, limit of detection was at least 30 times lower than the therapeutic concentration of ASA. The percent of relative standard deviation for determination of 10 replicates at level of 28.8 µg mL-1 of ASA was 5.8% and the minimum sampling rate was 30 samples per hour. Effects of some ingredients were investigated and the proposed method was applied successfully for the determination of ASA in pharmaceutical formulations and plasma samples. A time resolve CL approach was used to reduce the effect of interferences from cysteine and ascorbic acid in plasma samples. The possible CL reaction mechanism was also discussed.

【 授权许可】

Unknown