Fast Determination of Iron and Zinc in Hair and Human Serum Samples After Alkaline Solubilization by GF AAS

Journal of the Brazilian Chemical Society

Universidade Federal de Minas Gerais, Belo Horizonte-, Brazil¹  Silva, José Bento B. da¹  Beinner, Mark A.¹  Universidade Federal de Minas Gerais, Belo Horizonte, Brazil¹  Donnici, Cláudio L.¹  Souza, Carolina C.¹ 

关键词:  Universol®;    graphite furnace atomic absorption spectrometry;    iron;    zinc;    hair;    serum;    permanent chemical modifiers;   

DOI  :  10.5935/0103-5053.20150259

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Methods for the development and validation for determination of iron and zinc in human serum and hair samples by graphite furnace atomic absorption spectrometry (GF AAS) were performed. Solubilization was immediate by manual agitation in both samples with a 10 mL volume sample plus deionized water. Optimum pyrolysis and atomization temperatures were obtained by pyrolysis and atomization temperature curves in both matrices. For serum samples, the best temperatures were 1400 and 2500 °C (Fe) and 700 and 1600 °C (Zn), respectively. For hair samples, the best temperatures were 1000 and 2400 °C (Fe) and 800 and 1600 °C (Zn), respectively. Permanent modifiers and zirconium presented best the results for Zn in both matrices and for Fe in serum. Permanent modifier Nb was best for hair Zn. Serum and hair values were in agreement with the certified values for analytes and certified samples. The determined values for serum Fe and hair certified samples were 1.40 ± 0.2 and 114 ± 18 mg L-1, respectively. For Zn certified reference materials (CRMs), the certified samples values were 1720 ± 32 µg L-1 and 172 ± 5 mg kg-1. Simple, accurate and precise, this method represents a cost-effective detection protocol suitable for sample analysis for diagnosis of micronutrient malnutrition.

【 授权许可】

Unknown