Bioactive Cyanopeptides Produced by Sphaerocavum brasiliense Strains (Cyanobacteria)

Journal of the Brazilian Chemical Society

Bioalgas, São Paulo, Brazil¹  Sant’Anna, Célia Leite¹  Marli Fátima Fiore, Célia Leite¹  Universidade de São Paulo, Piracicaba, Brazil¹  Azevedo, Maria Teresa Paiva¹  Silva-Stenico, Maria Estela¹  Instituto de Botânica do Estado de São Paulo, São Paulo, Brazil¹  Lorenzi, Adriana Sturion¹  Rigonato, Janaina¹ 

关键词:  aeruginosin;    cyanopeptolin;    protease inhibitor;    mass spectrometry;    Allium test;    cyanobacteria;    Sphaerocavum genus;   

DOI  :  10.5935/0103-5053.20150194

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

The halogenated compounds aeruginosin and cyanopeptolin (protease inhibitors) are well documented in several cyanobacteria. In this work, the presence of genetic coding for aeruginosin and cyanopeptolin in Sphaerocavum genus and their characterization by mass spectrometry and potential toxic effects were investigated. Three strains of Sphaerocavum brasiliense (CCIBt 3094, CCIBt 3096 and CCIBt 3316) isolated from different reservoirs in São Paulo State, and one (CCIBt 3179) from Rio Grande do Norte State were selected for this study. Fragments of aerA-aerB and mcnC-mcnE gene regions (aeruginosin and cyanopeptolin, respectively) were amplified by polymerase chain reaction (PCR) and sequenced. Compounds were characterized by mass spectrometry. All the studied strains presented aeruginosin and cyanopeptolin synthetase genes. Mass spectrometry showed the presence of several aeruginosin and cyanopeptolin variants. The Allium test showed that the ethanolic extract promoted toxic and cytotoxic actions in acute and chronic treatments. These naturally occurring protease inhibitors can play an important role in the physiopathology of human diseases.

【 授权许可】

Unknown