Electrochemical reduction and stripping voltammetric determination of the anti-glaucoma drug levobunolol HCl in formulation and human serum at the mercury electrode

Journal of the Brazilian Chemical Society

Ghoneim, Ahmed M.¹  Tanta University, Tanta, Egypt¹  Abdel-Azzem, Magdi K.¹  Tanta University¹  Khattab, Ahmed E.¹  Monoufia University, Shebin Elkoom, Egypt¹  El-Desoky, Hanaa S.¹  Ghoneim, Mohamed M.¹ 

关键词: levobunolol HCl;    electrode reaction;    determination;    stripping voltammetry;   

DOI  :  10.5935/0103-5053.20140123

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Levobunolol HCl is a potent non-selective ß-adrenoceptor blocking agent used for the topical treatment of increased intraocular pressure in patients with chronic open angle glaucoma or ocular hypertension. Precise, rapid and extraction-free square-wave adsorptive cathodic stripping voltammetry (SW-AdCSV) method has been described for trace quantitation of levobunolol HCl in bulk form, commercial formulation (ophthalmologic drops) and human serum. Limits of quantification (LOQ) of 1.0 × 10–10 mol L–1 (in bulk form) and 2.5 × 10–10 mol L–1 levobunolol HCl (in spiked human serum) were achieved by the described method. Insignificant interferences from excipients associated with formulation of levobunolol HCl and from some common metal ions, co-administrated drugs, some other ß-blocker agents and its metabolite dihydrolevobunolol that are likely to be present in the biological fluids were obtained. The described SW-AdCSV method is sensitive enough to assay the drug in human serum compared to most of the reported methods.

【 授权许可】

Unknown