A comparison of plating and reverse transcriptase polymerase chain reaction followed by microchip electrophoresis for the inactivation of Alicyclobacillus acidoterrestris using saponin

Journal of the Brazilian Chemical Society

University of São Paulo, São Carlos, Brazil¹  Funes-Huacca, Maribel E.¹  Alberice, Juliana V.¹  University of Campinas, Campinas, Brazil¹  Carrilho, Emanuel¹ 

关键词: Alicyclobacillus acidoterrestris;    inactivation;    plating;    RT-PCR;    microchip;   

DOI  :  10.5935/0103-5053.20130272

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

It was used reverse-transcriptase polymerase chain reaction (RT-PCR) followed by capillary electrophoresis on a microchip to probe the viability of Alicyclobacillus acidoterrestris spores after inactivation with saponin and heat. The method was shown to be suitable for the purpose, and was faster and more sensitive than the traditional plating technique, the standard of the food industry. The limits of quantification and detection were 0.0107 and 0.0039 ng µL-1 of amplified DNA, respectively. The correlation coefficient (r) between traditional plating and our method was 0.9977, which indicates an excellent correspondence between them. Consequently, it was possible to assess, with confidence, the viability of bacteria using the RT-PCR reaction for detection. It was evaluated the potential of this molecular method over traditional microbiology in the inactivation of A. acidoterrestris by saponin as an effective agent, potentiated by heat.

【 授权许可】

Unknown